You_want_to_play_lets_play -

Fans often joke about his heavy French accent, spelling the line phonetically or swapping it with "You want to slay? Let's slay!"

Heavily shared on platforms like Tenor to express readiness for a challenge or game. 🎮 Alternative Gaming Reference

If you are looking at a classic gaming format rather than a specific quote:

Creators frequently use the audio for transition videos, cosplay, and gameplay highlights.

A popularized format of videos or screenshots documenting a playthrough of a video game, heavily featuring continuous commentary by the gamer. Want to Play? Let's Play! - TikTok

The line has become heavily localized into online meme culture:

Tour de Force . Activating it summons a custom, powerful sniper rifle that kills enemies with a single direct shot.

He says "You want to play? Let's play!" to his allies, while enemies and the player hearing him will hear the French version: "Ils sont partis!" (They are gone!). 🎭 Pop Culture & Internet Usage